Illumina’s sequencing by synthesis (SBS) technology is the most successful and widely-adopted next-generation sequencing platform worldwide. TruSeq technology supports massively parallel sequencing using a proprietary reversible terminator-based method that enables detection of single bases as they are incorporated into growing DNA strands. A fluorescently-labeled terminator is imaged as each dNTP is added and then cleaved to allow incorporation of the next base. Since all four reversible terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias. The end result is true base-by-base sequencing that enables the industry’s most accurate data for a broad range of applications.
For more information, visit Illumina’s website or MiSeq-datasheet [PDF].
|Read length||Instrument run time|
reads x 1 lane
|150 bp single read
or paired end
|24-36 hours for both|
Transcript profiling and splice variant detection using mRNA-Seq.
Protein-DNA interaction studies using ChIP-Seq,
Whole-exome sequencing using gene capture technology.
Small gene panel sequencing using custom-made gene capture technology.
MicroRNA and small RNA profiling.
Whole-genome sequencing of human DNA.
Genome-wide cytosine methylation scanning using bisulfite converted DNA.